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Chromatrap/Chromatrap ChIP-seq Protein G/500190/1 Ea

Chromatrap/Chromatrap ChIP-seq Protein G/500190/1 Ea
  • Chromatrap/Chromatrap ChIP-seq Protein G/500190/1 Ea
商品介绍
KitContents:Chromatrap®ProteinGspincolumns(24),ColumnConditioningBuffer,WashBuffer1,WashBuffer2,WashBuffer3,1.3MGlycine,LysisBuffer,ChIP-SeqElutionBuffer,0.1MNaOH,5MNaCl,1MNaHCO3,Hypotonicbuffer,ProteaseInhibitorCocktail(PIC),ProteinaseKstopsolution,ProteinaseK,1.5mlCollectionTubes.DNApurificationcolumns(24),DNABindingBuffer,DNAWashBufferandDNAElutionBuffer.Thekitalsoincludesantibodyvalidationcontrols:H3K4me3andIgGalongwithaForwardandReverseprimersetforGAPDH(humanpromoterregion).

Chromatrap®’snewChIP-SeqkitutilisesthesolidstatetechnologytodeliverastreamlinedChIP-Seqprotocolfromsmallcellnumbersandlowchromatinconcentrations.

Specificallyadaptedforbroaderchromatinconcentrations,Chromatrap®ChIP-SeqnowcombinesthedynamicrangeofChromatrap®withthedownstreamanalysispowerofdeepsequencing.Thisallowsfaster,morereproducIBLegenomewideidentificationoftranscriptionfactorbindingsitesandspecificDNAassociatedproteinmodifications.Thekitisidealformoredifficultprimarycellsamplesandfortheenrichmentoflowabundanttranscriptionfactors.

TheChIP-Seqkitisourmoresensitivekitespeciallyforlowabundanttranscriptionfactors,andcanbeusedforbothqPCRandsequencing. ThiskitiscompatiblewithbothhighandlowchromatinloADIngswithanincreasedslurryvolumeof1mlofferinggreaterflexibilitywithmoredifficultsamples.Thiskitrequiresaclean-upstepbutduetotheincreasedbindingofthecolumnsufficientDNAisobtainedfromonecolumnwithouttheneedtopoolsamplesforlibrarypreparation.

  • CompatibleforbothqPCRandsequencingasadownstreamprocess
  • Hasawidedynamicrangesuitableforlowandhighchromatinloadings(1-50µg),whichallowsforbetterresultsfromsmallsamplesizesandprovidersgreaterflexibility
  • Allowsusertoperformupto24ChIPassaysfromcellcollectionthroughtoimmunoprecipitation,includingupto10chromatinsamplepreparations.
  • KitprovidesallthemajorcomponentsrequiredforperformingChIPassaysandDNApurificationcolumnstoobtainhighqualityDNAforNGSlibrarypreparation
  • Positiveandnegativeantibodyandprimersetsareprovidedinthekit

AvailableinenzymaticformatforshearingofDNAbyenzymaticdigestionanda96wellmicroplateformatforhighthroughputChIP-SEQ.

NotsureifourChIP-Seqkitistherightchoiceforyourresearch?FindoutwhichChromatrap®ChIPkitisbestsuitedforyoubelow...

品牌介绍

Chromatrap为Porvair Science公司旗下的产品。该品牌产品主要为ChIP相关试剂盒。Chromatrap® ChIP 测定技术比传统磁珠法的效率更高,原因是与蛋白质 A 或 G结合的固相多孔聚合物捕获染色质的效率更高。尽管同样是使用离心柱法,但其优势是琼脂糖珠或磁珠远不可相比的.

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