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Chromatrap/Chromatrap Enymatic ChIP qPCR Protein G/500168/1 Ea

Chromatrap/Chromatrap Enymatic ChIP qPCR Protein G/500168/1 Ea
  • Chromatrap/Chromatrap Enymatic ChIP qPCR Protein G/500168/1 Ea
商品介绍
KitContents:Chromatrap®ProteinGspincolumns(24),ColumnConditioningBuffer,WashBuffer1,WashBuffer2,WashBuffer3,1.3MGlycine,LysisBuffer,DigestionBuffer,EnzymaticStopSolution,Elutionsolution,0.1MNaOH,5MNaCl,1MNaHCO3,Hypotonicbuffer,ShearingCocktail,ProteaseInhibitorCocktail(PIC),ProteinaseKstopsolution,ProteinaseK,1.5mlCollectionTubes.

Chromatrap®EnzymaticChIPkitsforqPCRrapidlyimproveChIPusingtheiruniquepatentedtechnologystreamliningtheprocess,ensuringchromatinpreparationtoqPCRanalysiscanbeperformedinunder5hours.

Byavoidingtheuseofmagneticoragarosebeads,non-specificbackgroundiseliminatedtogetherwithreducedmanualhandlingerrorsandsubsequentsampleloss.OptimisedelutionbufferchemistryallowssamplestobeanalyseddirectlyinqPCRwithouttheneedforDNAcleanup,furtherreducingsampleloss.

TheEnzymaticChIPkitisaperfectalternativeforthosewhocannotgetaccesstoasonicatortosheartheDNArequiredforaChIPassay.TheChIPqPCRassayisashortconvenientassayforlowchromatinloADIngwithverysmallslurryvolumes(40µls)withnoDNAcleanupstepspriortoqPCR.ThiskitisdesignedforuseasqPCRasthedownstreamprocessonlyandhasawidedynamicrangesuitableforlowchromatinloadings(1µg-10µg)

  • Optimisedfor1000ngsamplesizesallowingforbetterresultsfromsmallsamplesizesandprovidesgreaterflexibilitythusmoreIPassayscanbeperformedfromjustonesample
  • ThekitcontainssufficientProtein-AorProtein-Gbasedspin-columns,buffersandreagentstoperform24chromatinimmunoprecipitationassaysandreagentsforenzymaticshearingofchromatin(upto10preparations)
  • Ahighthroughputformatisalsoavailableallowingformultiplesampletype,antibodyandgenetargetstobetestedinparallel

Premiumkitscontainingpositiveandnegativeantibodyandprimersetsareavailabletoprovideyouwithsatisfactionofchromatinpreparation.

NotsureifourEnzymaticChIP-qPCRkitistherightchoiceforyourresearch?FindoutwhichChromatrap®ChIPkitisbestsuitedforyoubelow...

品牌介绍

Chromatrap为Porvair Science公司旗下的产品。该品牌产品主要为ChIP相关试剂盒。Chromatrap® ChIP 测定技术比传统磁珠法的效率更高,原因是与蛋白质 A 或 G结合的固相多孔聚合物捕获染色质的效率更高。尽管同样是使用离心柱法,但其优势是琼脂糖珠或磁珠远不可相比的.

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