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Chromatrap/Chromatrap FFPE ChIP-seq Protein A/500235/1 Ea

Chromatrap/Chromatrap FFPE ChIP-seq Protein A/500235/1 Ea
  • Chromatrap/Chromatrap FFPE ChIP-seq Protein A/500235/1 Ea
商品介绍
KitContents:Chromatrap®ProteinAspincolumns(24),ParaffinRemovalSolution,ColumnConditioningBuffer,WashBuffer1,WashBuffer2,WashBuffer3,FFPEElutionsolution,5MNaCl,1MNaHCO3,FFPELysisBuffer,ProteaseInhibitorCocktail(PIC),ProteinaseKstopsolution,ProteinaseK,1.5mlCollectiontubes.DNApurificationcolumns(24),DNAbindingbuffer,DNAwashbufferandDNAelutionbuffer.

AsolidphasechromatinimmunoprecipitationassayfromchromatinextractedfromFormalinFixedParaffinEmbedded(FFPE)tissue.TheChromatrap®FFPEChIPkithasovercometheissuesoftenassociatedwithFFPEChIP,byofferingastreamlinedprotocolwhichprovidestheuserwithgoodqualityisolatedchromatin.

Idealfortissuesamples,biopsiesanddifficulttolysesamples. Thesensitivityoftheuniquesolidstateplatformensuresthatsmallsamplescanbeusedwithefficientenrichmentofbothhighandlowabundanttargets.Chromatrap®FFPEChIPkitsprovideaquicker,easierandmoreefficientwayofperformingChIPassaysfromarangeofFFPEsamples.Usingtheunique,patentedChromatrap®technologymaximisesthecaptureefficiencyofthetargetchromatin/antibodycomplex.Byavoidingtheuseofmagneticoragarosebeads,non-specificbackgroundiseliminatedtogetherwithreducedmanualhandlingerrorsandsubsequentsampleloss. 

  • ThiskitiscompatIBLewithbothqPCRandsequencingasadownstreamprocessandhasawidedynamicrange,ensuringthatadequateDNAisenrichedforlibrarypreparationforsequencing.Thewidedynamicrangeallowsforbetterresultsfromsmallsamplesizesandprovidesgreaterflexibility
  • Priortosequencing,DNApurificationisrequiredandChromatrap®purificationcolumnsaresuppliedforeaseofuse.
  • ThekitscontainsufficientProteinAorProteinGbasedspin-columns,buffersandreagentstoperform24chromatinimmunoprecipitationassaysandupto10chromatinpreparations.

NotsureifourFFPEChIPkitistherightchoiceforyourresearch?FindoutwhichChromatrap®ChIPkitisbestsuitedforyoubelow...

品牌介绍

Chromatrap为Porvair Science公司旗下的产品。该品牌产品主要为ChIP相关试剂盒。Chromatrap® ChIP 测定技术比传统磁珠法的效率更高,原因是与蛋白质 A 或 G结合的固相多孔聚合物捕获染色质的效率更高。尽管同样是使用离心柱法,但其优势是琼脂糖珠或磁珠远不可相比的.

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